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cd34 antibody, anti-human, readye_lease  (Miltenyi Biotec)


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    Miltenyi Biotec cd34 antibody, anti-human, readye_lease
    Cd34 Antibody, Anti Human, Readye Lease, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+cd34/CD34+Antibody%2C+anti-human%2C+REAdye_lease/custom%40130-132-028%4042502392
    Average 94 stars, based on 1 article reviews
    cd34 antibody, anti-human, readye_lease - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Purification:

    Article Title: Mutation accumulation in cancer genes relates to nonoptimal outcome in chronic myeloid leukemia
    Article Snippet: .. CD34 + cells and CD3 + cells were purified with human CD34 + and CD3 + cell isolation kits (Miltenyi Biotec, Bergisch Gladbach, Germany). .. The purity of isolated cells was evaluated by flow cytometry with CD34-fluorescien isothiocyanate and CD3-allophycocyanin staining (BD Biosciences, San Jose, CA).

    Article Title: Correction of β-thalassemia by CRISPR/Cas9 editing of the α-globin locus in human hematopoietic stem cells
    Article Snippet: Peripheral blood was stained and red blood cells lysed during sample fixation (VersaLyse Lysing Solution and IOTest3 Fixative Solution; Beckman Coulter). .. Human CD34 + or CD45 + cells were purified from mouse peripheral blood or bone marrow by immunomagnetic selection with CD34 or CD45 MicroBead Kit UltraPure in combination with AUTOMACS PRO (PosselD2 Separation Program; Miltenyi Biotec). .. Statistical analyses were performed using GraphPad Prism, version 6.00, for Windows (GraphPad Software, La Jolla, CA).

    Cell Isolation:

    Article Title: Mutation accumulation in cancer genes relates to nonoptimal outcome in chronic myeloid leukemia
    Article Snippet: .. CD34 + cells and CD3 + cells were purified with human CD34 + and CD3 + cell isolation kits (Miltenyi Biotec, Bergisch Gladbach, Germany). .. The purity of isolated cells was evaluated by flow cytometry with CD34-fluorescien isothiocyanate and CD3-allophycocyanin staining (BD Biosciences, San Jose, CA).

    Modification:

    Article Title: Redirection to the bone marrow improves T cell persistence and antitumor functions
    Article Snippet: .. The murine A20 B-lymphoblastic cell line has previously been described ( ); in some experiments, the cell line was modified by pMP71 HuCD34 retroviral transduction to express human CD34 (A20.hCD34 + ) followed by isolation and sequential immunomagnetic enrichment using a human CD34 microbead kit (Miltenyi Biotec, Germany). ..

    Retroviral:

    Article Title: Redirection to the bone marrow improves T cell persistence and antitumor functions
    Article Snippet: .. The murine A20 B-lymphoblastic cell line has previously been described ( ); in some experiments, the cell line was modified by pMP71 HuCD34 retroviral transduction to express human CD34 (A20.hCD34 + ) followed by isolation and sequential immunomagnetic enrichment using a human CD34 microbead kit (Miltenyi Biotec, Germany). ..

    Transduction:

    Article Title: Redirection to the bone marrow improves T cell persistence and antitumor functions
    Article Snippet: .. The murine A20 B-lymphoblastic cell line has previously been described ( ); in some experiments, the cell line was modified by pMP71 HuCD34 retroviral transduction to express human CD34 (A20.hCD34 + ) followed by isolation and sequential immunomagnetic enrichment using a human CD34 microbead kit (Miltenyi Biotec, Germany). ..

    Isolation:

    Article Title: Redirection to the bone marrow improves T cell persistence and antitumor functions
    Article Snippet: .. The murine A20 B-lymphoblastic cell line has previously been described ( ); in some experiments, the cell line was modified by pMP71 HuCD34 retroviral transduction to express human CD34 (A20.hCD34 + ) followed by isolation and sequential immunomagnetic enrichment using a human CD34 microbead kit (Miltenyi Biotec, Germany). ..

    Staining:

    Article Title: The differential role of the lipid raft-associated protein flotillin 2 for progression of myeloid leukemia
    Article Snippet: .. Cells were then stained with an antibody to human CD34+ (130- 113-179, Miltenyi Biotec, Bergisch Gladbach, Germany) and sorted by flow cytometry. .. Normal human hematopoietic stem cells (HSC) (human) were CD34+ selected using magnetic beads and MACS separation according to the manufacturer’s specifications (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Defects in Long-Term APC Repopulation Ability of Adult Human Bone Marrow Hematopoietic Stem Cells (HSCs) Compared with Fetal Liver HSCs.
    Article Snippet: .. Fc receptors in single-cell suspensions were blocked with anti-mouse Fc receptor mAb (2.4G2) and 10% human AB serum (Gemini) and stained with fluorophore-conjugated Abs against mouse TER119 (Ter119), mouse CD45 (30-F11), human CD45 (H130), HLA-DR (G46-6), mouse/human CD11b (M1/70), mouse/human CD11c (B-ly6), human CD123 (9F5) (from BD Pharmingen); human CD45RA (MEM-56), human CD14 (61D3), human CD73 (AD2), human CD90 (5E10), human CD105 (SN6), human CD34 (581), human CD38 (HIT2), human CD10 (HI10a), human CD19 (HIB19), human CD115 (9- 4D2-1E4), human CD123 (6H6) (from eBioscience); and anti-human CD303 (AC144) (from Miltenyi Bioscience). ..

    Flow Cytometry:

    Article Title: The differential role of the lipid raft-associated protein flotillin 2 for progression of myeloid leukemia
    Article Snippet: .. Cells were then stained with an antibody to human CD34+ (130- 113-179, Miltenyi Biotec, Bergisch Gladbach, Germany) and sorted by flow cytometry. .. Normal human hematopoietic stem cells (HSC) (human) were CD34+ selected using magnetic beads and MACS separation according to the manufacturer’s specifications (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Selection:

    Article Title: PCDH17 functions as a common tumor suppressor gene in acute leukemia and its transcriptional downregulation is mediated primarily by aberrant histone acetylation, not DNA methylation.
    Article Snippet: We recently reported that methylation of PCDH17 gene is found in 30% of children with B-cell precursor acute lymphoblastic leukemia (ALL), and is significantly correlated to event-free or overall survival.. We here evaluated PCDH17 mRNA expression in pediatric acute myeloid leukemia (AML) and ALL.. PCDH17 mRNA expression levels in children with ALL/AML were lower than those in healthy counterparts.

    Article Title: Dual-targeting triplebody 33-16-123 (SPM-2) mediates effective redirected lysis of primary blasts from patients with a broad range of AML subtypes in combination with natural killer cells
    Article Snippet: Prior to use in cytolysis experiments, the cells were thawed and cultured overnight in RPMI medium containing 5% human serum (Invitrogen) plus 50 units/ml and 50 μg/ml PS, respectively, but no additional IL-2. .. Enrichment of human CD34-positive cells by preparative sorting with immuno-magnetic beads CD34-positive cells were enriched by positive selection using the commercial human CD34+ Multisort Kit (Miltenyi Biotec MACS sorting kit, cat. #130–058-701) according to manufacturer’s instructions. ..

    Article Title: Correction of β-thalassemia by CRISPR/Cas9 editing of the α-globin locus in human hematopoietic stem cells
    Article Snippet: Peripheral blood was stained and red blood cells lysed during sample fixation (VersaLyse Lysing Solution and IOTest3 Fixative Solution; Beckman Coulter). .. Human CD34 + or CD45 + cells were purified from mouse peripheral blood or bone marrow by immunomagnetic selection with CD34 or CD45 MicroBead Kit UltraPure in combination with AUTOMACS PRO (PosselD2 Separation Program; Miltenyi Biotec). .. Statistical analyses were performed using GraphPad Prism, version 6.00, for Windows (GraphPad Software, La Jolla, CA).

    Magnetic Cell Separation:

    Article Title: Dual-targeting triplebody 33-16-123 (SPM-2) mediates effective redirected lysis of primary blasts from patients with a broad range of AML subtypes in combination with natural killer cells
    Article Snippet: Prior to use in cytolysis experiments, the cells were thawed and cultured overnight in RPMI medium containing 5% human serum (Invitrogen) plus 50 units/ml and 50 μg/ml PS, respectively, but no additional IL-2. .. Enrichment of human CD34-positive cells by preparative sorting with immuno-magnetic beads CD34-positive cells were enriched by positive selection using the commercial human CD34+ Multisort Kit (Miltenyi Biotec MACS sorting kit, cat. #130–058-701) according to manufacturer’s instructions. ..

    Single Cell:

    Article Title: Defects in Long-Term APC Repopulation Ability of Adult Human Bone Marrow Hematopoietic Stem Cells (HSCs) Compared with Fetal Liver HSCs.
    Article Snippet: .. Fc receptors in single-cell suspensions were blocked with anti-mouse Fc receptor mAb (2.4G2) and 10% human AB serum (Gemini) and stained with fluorophore-conjugated Abs against mouse TER119 (Ter119), mouse CD45 (30-F11), human CD45 (H130), HLA-DR (G46-6), mouse/human CD11b (M1/70), mouse/human CD11c (B-ly6), human CD123 (9F5) (from BD Pharmingen); human CD45RA (MEM-56), human CD14 (61D3), human CD73 (AD2), human CD90 (5E10), human CD105 (SN6), human CD34 (581), human CD38 (HIT2), human CD10 (HI10a), human CD19 (HIB19), human CD115 (9- 4D2-1E4), human CD123 (6H6) (from eBioscience); and anti-human CD303 (AC144) (from Miltenyi Bioscience). ..



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    Miltenyi Biotec cd34 microbead kit
    ZEB1 is required for optimal TED in primary human cells. ( A ) Experimental scheme of ZEB1 KD in primary human <t>CD34</t> + HSPCs. ( B ) ZEB1 mRNA levels measured at day 3 of differentiation. ( C ) GPA levels at day 3 of erythroid differentiation. Percentages of GPA + cells are indicated. ( D ) Cell growth rates at days 3, 7, and 10. ( E ) Proportion of erythroid progenitors, pro-erythroblasts (Pro-E), and basophilic erythroblasts (Baso) at day 3 of erythroid differentiation. ( F ) mRNA levels of GATA2 and KLF1 in ZEB1 KD cells (day 3). ( G ) Enucleation rates measured by Syto-60 staining at day 10 of differentiation. Data are mean ± SEM of n = 3–5 independent biological experiments, from three independent blood donors, * P < .05, ** P < .01, *** P < .001, **** P < .0001 (Student’s t -test).
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    Image Search Results


    B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed progenitor cells (A) Flow cytometric analysis of CD34, c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .

    Journal: Cell Reports Medicine

    Article Title: ABO: A 3D stroma-supported culture platform enabling full human B-lymphopoiesis for disease modeling and gene therapy development

    doi: 10.1016/j.xcrm.2026.102879

    Figure Lengend Snippet: B cell development in ABOs is not driven by expansion of pre-existing B-lineage-committed progenitor cells (A) Flow cytometric analysis of CD34, c-KIT (CD117), CD38, and CD10 expression on HSPCs before (top) and after depletion of Lin + CD10 + cells (bottom) by MACS, visualized with opt-SNE. (B and C) Flow cytometric analysis comparing the frequencies of CD33 + myeloid-committed (B) and CD10 + CD38 + lymphoid-committed cells (C) in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (D) Quantification of CD33 + and CD10 + CD38 + cells in day 35 ABOs. (E) Flow cytometric analysis comparing IgM + IgD − (immature) and IgM + IgD + (transitional) B cell frequencies in day 35 ABOs using bulk or Lin + CD10 + -depleted CD34 + HSPCs. (F) Quantification of IgM + and IgM + IgD + B cell frequencies in day 35 ABOs. (G) Distribution of HSPCs along the B cell developmental trajectory in day 35 ABOs. (D, F, G) Data are presented as mean ± SEM. Statistical analysis was performed using two-way ANOVA followed by Bonferroni’s multiple comparisons test (ns; non-significant). (A–G) Data represent n = 3 individual donors in independent cultures. See also .

    Article Snippet: Leukocytes from UCB and mPB were first purified using the Erythrocyte Sedimentation Kit II (Miltenyi), followed by CD34 + cell enrichment with the CD34 MicroBead Kit UltraPure (Miltenyi) per manufacturer’s instructions.

    Techniques: Expressing

    NOX1i in Myeloid-HIS-HCC mice reduces monocyte and tumor-associated macrophage infiltration. (A) Schematic overview of the experimental set-up. Male NSG-SGM3 mice (n = 11) were sublethally irradiated and the immune system was partly reconstituted using human cord blood (CB)-derived hematopoietic stem and progenitor cells (HSPCs). Four weeks post humanization, orthotopic human HCC was induced and mice were treated with 50 µM ML171 (NOX1i; n = 6) or vehicle (n = 5), twice per week for 3 weeks. (B) Human-to-murine immune cell (CD45) chimerism in the indicated tissues. (C) Human engraftment based on the number of human CD45 cells. Data are represented as violin plots showing median and quartiles. (D,E) Human immune cell subsets (D) and monocyte subsets (E) in indicated tissues represented as percentage of human CD45 cells. (F) Number of human macrophages and monocytes in the indicated tissues. (G) Representative immunohistochemistry images of infiltrated human monocytes/macrophages in liver tissue of HCC-bearing humanized mice. Sections were stained for human CD14 (brown), or matched IgG control. Scale bars: 100 µm. (B,D,E) . Data are shown as mean (SD). (C–E) Adjusted p-values were calculated using multiple unpaired t-test with Holm-Šídák correction (D,E) , or p-values were calculated using unpaired t-test with Welch’s correction or unpaired Mann-Whitney U test based on normal distribution (C,E) . *p < 0.05. HCC: hepatocellular carcinoma, NOX1i: NOX1 inhibition, huCD45: human CD45, muCD45: murine CD45, NK = natural killer, DCs: dendritic cells.

    Journal: Frontiers in Pharmacology

    Article Title: Harnessing human immune system models to validate NADPH oxidase 1 inhibition as treatment for hepatocellular carcinoma

    doi: 10.3389/fphar.2026.1808515

    Figure Lengend Snippet: NOX1i in Myeloid-HIS-HCC mice reduces monocyte and tumor-associated macrophage infiltration. (A) Schematic overview of the experimental set-up. Male NSG-SGM3 mice (n = 11) were sublethally irradiated and the immune system was partly reconstituted using human cord blood (CB)-derived hematopoietic stem and progenitor cells (HSPCs). Four weeks post humanization, orthotopic human HCC was induced and mice were treated with 50 µM ML171 (NOX1i; n = 6) or vehicle (n = 5), twice per week for 3 weeks. (B) Human-to-murine immune cell (CD45) chimerism in the indicated tissues. (C) Human engraftment based on the number of human CD45 cells. Data are represented as violin plots showing median and quartiles. (D,E) Human immune cell subsets (D) and monocyte subsets (E) in indicated tissues represented as percentage of human CD45 cells. (F) Number of human macrophages and monocytes in the indicated tissues. (G) Representative immunohistochemistry images of infiltrated human monocytes/macrophages in liver tissue of HCC-bearing humanized mice. Sections were stained for human CD14 (brown), or matched IgG control. Scale bars: 100 µm. (B,D,E) . Data are shown as mean (SD). (C–E) Adjusted p-values were calculated using multiple unpaired t-test with Holm-Šídák correction (D,E) , or p-values were calculated using unpaired t-test with Welch’s correction or unpaired Mann-Whitney U test based on normal distribution (C,E) . *p < 0.05. HCC: hepatocellular carcinoma, NOX1i: NOX1 inhibition, huCD45: human CD45, muCD45: murine CD45, NK = natural killer, DCs: dendritic cells.

    Article Snippet: Freshly isolated CB-derived PBMCs, isolated from CB as described for buffy coat, were enriched for HSPCs based on positive immunomagnetic selection of CD34 + expressing cells using the human CD34 MicroBead Kit UltraPure (Miltenyi Biotec, #130–100-453) according to manufacturer’s guidelines.

    Techniques: Irradiation, Derivative Assay, Immunohistochemistry, Staining, Control, MANN-WHITNEY, Inhibition

    ZEB1 is required for optimal TED in primary human cells. ( A ) Experimental scheme of ZEB1 KD in primary human CD34 + HSPCs. ( B ) ZEB1 mRNA levels measured at day 3 of differentiation. ( C ) GPA levels at day 3 of erythroid differentiation. Percentages of GPA + cells are indicated. ( D ) Cell growth rates at days 3, 7, and 10. ( E ) Proportion of erythroid progenitors, pro-erythroblasts (Pro-E), and basophilic erythroblasts (Baso) at day 3 of erythroid differentiation. ( F ) mRNA levels of GATA2 and KLF1 in ZEB1 KD cells (day 3). ( G ) Enucleation rates measured by Syto-60 staining at day 10 of differentiation. Data are mean ± SEM of n = 3–5 independent biological experiments, from three independent blood donors, * P < .05, ** P < .01, *** P < .001, **** P < .0001 (Student’s t -test).

    Journal: Nucleic Acids Research

    Article Title: ZEB1 drives terminal erythroid maturation by controlling the GATA2–KLF1 regulatory switch

    doi: 10.1093/nar/gkag613

    Figure Lengend Snippet: ZEB1 is required for optimal TED in primary human cells. ( A ) Experimental scheme of ZEB1 KD in primary human CD34 + HSPCs. ( B ) ZEB1 mRNA levels measured at day 3 of differentiation. ( C ) GPA levels at day 3 of erythroid differentiation. Percentages of GPA + cells are indicated. ( D ) Cell growth rates at days 3, 7, and 10. ( E ) Proportion of erythroid progenitors, pro-erythroblasts (Pro-E), and basophilic erythroblasts (Baso) at day 3 of erythroid differentiation. ( F ) mRNA levels of GATA2 and KLF1 in ZEB1 KD cells (day 3). ( G ) Enucleation rates measured by Syto-60 staining at day 10 of differentiation. Data are mean ± SEM of n = 3–5 independent biological experiments, from three independent blood donors, * P < .05, ** P < .01, *** P < .001, **** P < .0001 (Student’s t -test).

    Article Snippet: CD34 + were isolated by positive selection using the CD34 MicroBead Kit (MACS Miltenyi) according to the manufacturers’ recommendation and cultured following a human ex vivo differentiation protocol as previously described [ ].

    Techniques: Staining